Herpes simplex virus I+II (HSVI+II) Antibody (IgG) BioAssay(TM) ELISA Kit (Human)

Catalog Number: USB-351649
Article Name: Herpes simplex virus I+II (HSVI+II) Antibody (IgG) BioAssay(TM) ELISA Kit (Human)
Biozol Catalog Number: USB-351649
Supplier Catalog Number: 351649
Alternative Catalog Number: USB-351649-48,USB-351649-96
Manufacturer: US Biological
Category: Kits/Assays
Application: ELISA
Sample Type: Serum Intended Use: For the qualitative determination of human herpes simplex virus I+II (HSVI+II) antibody (IgG) concentrations in serum. Specificity: This assay has high sensitivity and excellent specificity for detection of human HSV I+II antibody (IgG). No significant cross-reactivity or interference between human HSVI+II antibody (IgG) and analogues was observed. Test Principle: This BioAssay(TM) employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human HSVI+II antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Coated assay plate, 1x96 wells Negative Control, 1x300ul Positive Control, 1x300ul Sample Diluent, 1x12ml HRP-conjugate, 1x12ml Wash Buffer (30X), 1x30ml Substrate A, 1x6ml Substrate B, 1x6ml Stop Solution, 1x6ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.