Immunoglobulin A (IgA) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Immunoglobulin A (IgA) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-351651
• Supplier Catalog Number: 351651
• Alternative Catalog Number: USB-351651-48,USB-351651-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Sample Type: Serum and plasma Intended Use: For the quantitative determination of human immunoglobulin A (IgA) concentrations in serum, plasma. Sensitivity: 1.56ng/ml Range: 0.25-1000ng/ml Specificity: This assay has high sensitivity and excellent specificity for detection of human IgA. No significant cross-reactivity or interference between human IgA and analogues was observed. Test Principle: This BioAssay(TM) employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgA present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for IgA is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgA bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate (12x8 coated microwells), 1x96 wells Standard (Freeze dried), 2x vials HRP-conjugate (100X), 1x120ul HRP-conjugate Diluent, 1x20ml Sample Diluent, 2x20ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.