Influenza A Virus (FluA) Antibody (IgM) BioAssay(TM) ELISA Kit (Human)

Catalog Number: USB-351653
Article Name: Influenza A Virus (FluA) Antibody (IgM) BioAssay(TM) ELISA Kit (Human)
Biozol Catalog Number: USB-351653
Supplier Catalog Number: 351653
Alternative Catalog Number: USB-351653-48,USB-351653-96
Manufacturer: US Biological
Category: Kits/Assays
Application: ELISA
Sample Type: Serum Intended Use: For the qualitative determination of human influenza A virus (FluA) antibody (IgM) concentrations in serum. Specificity: This assay has high sensitivity and excellent specificity for detection of human influenza A virus (FluA) antibody (IgM). No significant cross-reactivity or interference between human influenza A virus (FluA) antibody (IgM) and analogues was observed. Test Principle: This BioAssay(TM) employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Ag. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibody specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human influenza A virus (FluA) antibody (IgM) bound in the intial step. The color development is stopped and the intensity of the color is measured. Kit Components: Coated assay plate, 1x96 wells Negative Control, 1x1ml Positive Control, 1x1ml HRP-conjugate, 2x5ml Sample Diluent, 1x20ml Wash Buffer (25X), 1x20ml Substrate A, 1x5ml Substrate B, 1x5ml Stop Solution, 1x5ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.