Insulin Autoantibody (IAA) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Insulin Autoantibody (IAA) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-351655
• Supplier Catalog Number: 351655
• Alternative Catalog Number: USB-351655-48,USB-351655-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Sample Type: Serum and plasma Intended Use: For the qualitative determination of human insulin autoantibodies (IAA) in serum and plasma. Specificity: This assay has high sensitivity and excellent specificity for detection of human IAA. No significant cross-reactivity or interference between human IAA and analogues was observed. Test Principle: This BioAssay(TM) employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with purified antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human IAA bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Coated assay plate, 1x96 wells Negative Control, 1x1ml Positive Control, 1x1ml HRP-conjugate, 1x12ml Sample Diluent, 1x12ml Wash Buffer (20X), 1x30ml Substrate A, 1x7ml Substrate B, 1x7ml Stop Solution, 1x7ml Adhesive Strip (For 96 wells), 4x strips Instruction manual, 1 Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.