Platelet-Derived Growth Factor-BB (PDGF-BB) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Platelet-Derived Growth Factor-BB (PDGF-BB) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-351686
• Supplier Catalog Number: 351686
• Alternative Catalog Number: USB-351686-48,USB-351686-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Sample Type: Serum, plasma, tissue homogenates Intended Use: For the quantitative determination of human platelet-derived growth factor-BB (PDGF-BB) concentrations in serum, plasma and tissue homogenates. Sensitivity: 3.12pg/ml Range: 31.2-2000pg/ml Specificity: This assay has high sensitivity and excellent specificity for detection of human PDGF-BB. No significant cross-reactivity or interference between human PDGF-BB and analogues was observed. Test Principle: This BioAssay(TM) employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PDGF-BB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDGF-BB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDGF-BB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate (12x8 coated microwells), 1x96 wells Standard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.