Rotavirus (RV) antigen (Ag) BioAssay(TM) ELISA Kit (Porcine)

Product Overview

• Article Name: Rotavirus (RV) antigen (Ag) BioAssay(TM) ELISA Kit (Porcine)
• Biozol Catalog Number: USB-351692
• Supplier Catalog Number: 351692
• Alternative Catalog Number: USB-351692-48,USB-351692-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Sample Type: Feces Intended Use: For the qualitative determination of porcine rotavirus (RV) antigen (Ag) concentrations in feces. Test Principle: This BioAssay(TM) employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rotavirus antigen. Samples are pipetted into the wells with antibody conjugated Horseradish Peroxidase(HRP). Any Rotavirus antigen present in samples will bind to the pre-coated specific antibody. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human Rotavirus antigen bound in the initial step. The color development is stopped and the intensity of the color is measured. Specificity: This assay has high sensitivity and excellent specificity for detection of porcine Rotavirus antigen. No significant cross-reactivity or interference between porcine Rotavirus antigen and analogues was observed. Kit Components: Assay plate, 1x96 wells Negative Control, 1x800ul Positive Control, 1x800ul HRP-conjugate, 1x6ml Wash Buffer, 1x6ml Substrate A, 1x5ml Substrate B, 1x5ml Stop Solution, 1x5ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.