Sperm Antibody (AsAb) BioAssay(TM) ELISA Kit (Human)

Product Overview

• Article Name: Sperm Antibody (AsAb) BioAssay(TM) ELISA Kit (Human)
• Biozol Catalog Number: USB-351698
• Supplier Catalog Number: 351698
• Alternative Catalog Number: USB-351698-48,USB-351698-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Sample Type: Serum Intended Use: For the qualitative determination of human anti-sperm antibody (AsAb) concentrations in serum. Specificity: This assay has high sensitivity and excellent specificity for detection of human anti-sperm antibody (AsAb). No significant cross-reactivity or interference between human anti-sperm antibody (AsAb) and analogues was observed. Test Principle: This BioAssay(TM) employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human anti-sperm antibody (AsAb) bound in the initial step. The color development is stopped and the intensity of the color is measured. Sample Volume: 50-100ul Detection Wavelength: 450nm Precision: Intra-assay: CV% <15% Inter-assay: CV% <15% Kit Components: Coated assay plate, 1x96 wells Negative Control, 1x1ml Positive Control, 1x1ml Sample Diluent, 1x20ml HRP-conjugate, 1x10ml Wash Buffer (25X), 1x20ml Substrate A, 1x5ml Substrate B, 1x5ml Stop Solution, 1x5ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.