Apolipoprotein A-I, Recombinant, Human (ApoA-I, APOA1)

Product Overview

• Article Name: Apolipoprotein A-I, Recombinant, Human (ApoA-I, APOA1)
• Biozol Catalog Number: USB-352674
• Supplier Catalog Number: 352674
• Alternative Catalog Number: USB-352674-20,USB-352674-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

ApoA-I is a protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high density lipoprotein (HDL) particle. ApoA-I, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This ApoA-I ability enables HDL to remove excess peripheral cholesterol, and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is thought to inhibit atherogenesis. For this reason, HDL is also known as the good cholesterol. The therapeutic potential of ApoA-I has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of ApoA-I (called ApoA-I Milano). The availability of recombinant normal ApoA-I should facilitate further investigation into the potential usefulness of ApoA-I in preventing atherosclerotic vascular diseases. Recombinant human ApoA-I is a 28.2kD protein of 244 amino acid residues. Recombinant protein corresponding to human Apolipoprotein A-1, expressed in E. coli. Molecular Weight: ~28.2kD Amino Acid Sequence: MDEPPQSPWD RVKDLATVYV DVLKDSGRDY VSQFEGSALG KQLNLKLLDN WDSVTSTFSK LREQLGPVTQ EFWDNLEKET EGLRQEMSKD LEEVKAKVQP YLDDFQKKWQ EEMELYRQKV EPLRAELQEG ARQKLHELQE KLSPLGEEMR DRARAHVDAL RTHLAPYSDE LRQRLAARLE ALKENGGARL AEYHAKATEH LSTLSEKAKP ALEDLRQGLL PVLESFKVSF LSALEEYTKK LNTQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 28.2
• UniProt: P02647
• Purity: 97% (RP-HPLC, SDS-PAGE)
• Form: Supplied as a lyophilized powder in PBS, pH 7.0. No preservative added. Reconstitute with sterile ddH2O to 0.1mg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).