IGFBP2, BioAssay(TM) ELISA Kit (Mouse) (Insulin Like Growth Factor Binding Protein 2, IBP2)
Biozol Catalog Number:
USB-352917
Supplier Catalog Number:
352917
Alternative Catalog Number:
USB-352917-96
Manufacturer:
US Biological
Category:
Kits/Assays
Application:
ELISA
Insulin-like growth factor-binding protein 2 (IGFBP2), also called IBP2, is a protein that in humans is encoded by the IGFBP2 gene. It is mapped to 2q35. By structural analysis, it showed that the IGFBP2 gene consists of 4 exons with 3 introns of lengths 27.0, 1.0, and 1.9 kb. IGFBP2 secreted by metastatic cells recruits endothelia by modulating IGF1 mediated activation of the IGF type-I receptor on endothelial cells. The IGFBP2/IGF1/IGF1R and GAS6/MERTK signaling pathways are regulators of cancer-mediated endothelial recruitment. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA) Intended Use: For quantitative detection of mouse IGFBP2 in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <10pg/ml Range: 125-8000pg/ml Specificity: Natural and recombinant mouse IGFBP2 Test Principle: This mouse IGFBP2 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP2 has been precoated onto 96-well plates. Standards (NSO) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP2 amount of sample captured in plate. Kit Components: 96-well plate precoated with mouse IGFBP2 antibody, 1 Lyophilized recombinant mouse IGFBP2 standard, 10ng/tubex2 Biotinylated mouse IGFBP2 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
