IL1R2, BioAssay(TM) ELISA Kit (Human) (Interleukin 1 Receptor Type II, CD121b)
Biozol Catalog Number:
USB-352936
Supplier Catalog Number:
352936
Alternative Catalog Number:
USB-352936-96
Manufacturer:
US Biological
Category:
Kits/Assays
Application:
ELISA
Interleukin 1 receptor, type II (IL1R2) also known as CD121b (Cluster of Differentiation 121b) is an interleukin receptor. The protein encoded by this gene is a cytokine receptor that belongs to the interleukin-1 receptor family. It is mapped to 2q11.2. IL1R2 binds interleukin alpha(IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I (IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 also modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. The secreted IL1R2 recruits secreted IL1RAP with high affinity, and this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors. Sample Type: Cell culture supernates and serum. Intended Use: For quantitative detection of human IL1R2 in cell culture supernates and serum. Sensitivity: <10pg/ml Range: 31.2-2000pg/ml Specificity: Natural and recombinant human IL1R2 Test Principle: This human IL1R2 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL1R2 has been precoated onto 96-well plates. Standards(sf21) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL1R2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL1R2 amount of sample captured in plate. Kit Components: 96-well plate precoated with human IL1R2 antibody, 1 Lyophilized recombinant human IL1R2 standard, 10ng/tubex2 Biotinylated human IL1R2 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex(ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
