Serum Amyloid A (SAA), BioAssay(TM) ELISA Kit (Mouse)

Product Overview

• Article Name: Serum Amyloid A (SAA), BioAssay(TM) ELISA Kit (Mouse)
• Biozol Catalog Number: USB-353034
• Supplier Catalog Number: 353034
• Alternative Catalog Number: USB-353034-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Serum amyloid A protein(SAA), also known as SAA1, is a protein that in humans is encoded by the SAA1 gene. This gene encodes a member of the serum amyloid A family of apolipoproteins. It is mapped to 11p15.1. SAA is a major acute phase protein that is highly expressed in response to inflammation and tissue injury. This protein also plays an important role in HDL metabolism and cholesterol homeostasis. High levels of this protein are associated with chronic inflammatory diseases including atherosclerosis, rheumatoid arthritis, Alzheimers disease and Crohns disease. This protein may also be a potential biomarker for certain tumors. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA) and urine Intended Use: For quantitative detection of mouse SAA in cell culture supernates, serum, plasma (heparin, EDTA) and urine. Sensitivity: <10pg/ml Range: 156-10,000pg/ml Specificity: Natural and recombinant mouse SAA Test Principle: This mouse SAA BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SAA has been precoated onto 96-well plates. Standards(E.coli) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAA is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SAA amount of sample captured in plate. Kit Components: 96-well plate precoated with mouse SAA antibody, 1 Lyophilized recombinant mouse SAA standard, 10ng/tube*2 Biotinylated mouse SAA antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.