SPARC Like Protein 1 (SPARCL1), BioAssay(TM) ELISA Kit (Human)
Biozol Catalog Number:
USB-353046
Supplier Catalog Number:
353046
Alternative Catalog Number:
USB-353046-96
Manufacturer:
US Biological
Category:
Kits/Assays
Application:
ELISA
SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a protein that in humans is encoded by the SPARCL1 gene. The cells in high endothelial venules (HEVs) in lymphoid tissues have a plump, almost cuboidal, appearance and support high levels of lymphocyte extravasation from blood, possibly due to the presence of desmosome-like junctions rather than tight junctions in the HEVs. In chronic inflammation, the activated endothelium of nonlymphoid tissues acquires an HEV-like morphology and function. SPARCL1 is highly expressed in HEV and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration. Sample Type: Cell culture supernates and serum. Intended Use: For quantitative detection of human SPARCL1 in cell culture supernates and serum. Sensitivity: <10pg/ml Range: 46.9-3000pg/ml Specificity: Natural and recombinant human SPARCL1 Test Principle: This human SPARCL1 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SPARCL1 has been precoated onto 96-well plates. Standards(NSO, I17-F664) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPARCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SPARCL1 amount of sample captured in plate. Kit Components: 96-well plate precoated with human SPARCL1 antibody, 1 Lyophilized recombinant human SPARCL1 standard, 10ng/tube*2 Biotinylated human SPARCL1 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
