FA/VB9 (Folic Acid/Vitamin B9) BioAssay(TM) ELISA Kit (Universal)

Product Overview

• Article Name: FA/VB9 (Folic Acid/Vitamin B9) BioAssay(TM) ELISA Kit (Universal)
• Biozol Catalog Number: USB-355547
• Supplier Catalog Number: 355547
• Alternative Catalog Number: USB-355547-48, USB-355547-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The FA/VB9 (Folic Acid/Vitamin B9) BioAssay(TM) ELISA Kit is a quantitative competitive assay for the detection of Vitamin B12 in serum, plasma, tissue homogenates and other biological fluids. Range: 12.5-800ng/ml Sensitivity: <7.5ng/ml Precision: Intra-assay CV: <8% Inter-assay CV: <10% Assay Principle: This ELISA kit employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with an antibody specific to FA/VB9. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to FA/VB9. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FA/VB9, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of FA/VB9 in the sample is then determined by an inverse correlation between FA/VB9 in the samples and the assay signal intensity. Kit Components: *355547A: Microtiter Strips, 1x96 wells (8x12 wells). *355547B: Standard, 2x1 vials 355547C: Sample/Standard Dilution Buffer, 1x20ml 355547D: Antibody (Biotin) (Concentrated), 1x60ul 355547E: Antibody Dilution Buffer, 1x10ml 355547F: Streptavidin (HRP) (SABC), 1x120ul 355547G: SABC Dilution Buffer, 1x10ml 355547H: TMB Substrate, 1x10ml 355547J: Stop Solution, 1x10ml 355547K: Wash Buffer, 25X, 1x30ml Storage and Stability: Store unopened *355547A at 4C, store at -20C once opened. Store unopened *355547B at 4C, once reconstituted, store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Wash plate 2 times before adding standards, samples and control (zero) to wells! 2. Add 50ul standard or sample to each well. Immediately add 50ul (Biotin) working solution to each well. 3. Incubate for 45 minutes at 37C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results.