PVRL3 (Poliovirus Receptor Related Protein 3, CDw113, CD113, PPR3, PRR3, PVRR3, Nectin-3), Rabbit
Biozol Catalog Number:
USB-364837
Supplier Catalog Number:
364837
Alternative Catalog Number:
USB-364837-200
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, IHC, WB
Immunogen:
Recombinant protein corresponding to Thr171-Ile377 of human PVRL3, expressed in E. coli.
Reymond et al. (2000) cloned a full-length cDNA encoding PRR3. The deduced 550aa PRR3 protein has a calculated molecular mass of 55.4kD and contains an N-terminal hydrophobic leader sequence, an ectodomain containing a V-type Ig domain and 2 C-type Ig domains bracketed by 6 cysteine residues, a hydrophobic transmembrane region, and a long cytoplasmic sequence. PRR3 also has 6 potential N-glycosylation sites. Northern blot analysis revealed PRR3 expression predominantly in placenta and testis, with faint or no expression in other tissues. A major transcript of 2.8 kb and minor transcripts of 9.5, 6.0, and 4.7 kb were detected in placenta. PRR3 was also expressed in mammalian epithelial cell lines. Flow cytometry showed expression of PRR3 at the surface of transfected COS-1 cells. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
