RFN55 (Ring Finger Protein 55, CBL, C-CBL, CBL2, Cas-Br-M(Murine)Ecotropic Retroviral Transforming Sequence, Casitas B-lineage Lymphoma, Proto-oncogene c-Cbl, Signal transduction protein CBL), Rabbit
Biozol Catalog Number:
USB-364898
Supplier Catalog Number:
364898
Alternative Catalog Number:
USB-364898-200
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, IHC, WB
Immunogen:
Recombinant protein corresponding to Ser558-Gly816 of human RFN55, expressed in E. coli.
The virus used in the experiment was a retrovirus known as Cas-Br-M, and was found to have excised approximately a third of the original c-Cbl gene from mice it was injected into. Sequencing revealed that the portion carried by the retrovirus encoded a tyrosine kinase binding domain, and that this was the oncogenic form as retroviruses carrying full-length c-Cbl did not induce tumor formation. The resultant transformed retrovirus was found to consistently induce a type of pre-B lymphoma, known as Casitas B-lineage lymphoma, in infected mice. This domain structure and the tyrosine and serine-rich content of the protein product is typical of an adaptor molecule used in cell signalling pathways. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
