RGS1 (Regulator Of G Protein Signaling 1, 1R20, BL34, IER1, IR20, B-cell activation protein BL34, Early response protein 1R20), Rabbit
Biozol Catalog Number:
USB-364903
Supplier Catalog Number:
364903
Alternative Catalog Number:
USB-364903-200
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, IHC, WB
Immunogen:
Recombinant protein corresponding to Met1-Lys209 of human RGS1, expressed in E. coli.
Regulator of G-protein signaling 1 is a member of the regulator of G-protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120aa motif called the RGS domain. The protein attenuates the signalling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. B-cell specific. Expression is relatively low in B-cells and chronic lymphocytic leukemia B-cells, however, in other types of malignant B-cell such as non-Hodgkins lymphoma and hairy cell leukemia, expression is constitutively high. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
