RGS19 (Regulator Of G Protein Signaling 19, GAIP, RGSGAIP, GNAI3IP, G-alpha-interacting protein), Rabbit
Biozol Catalog Number:
USB-364904
Supplier Catalog Number:
364904
Alternative Catalog Number:
USB-364904-200
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
ELISA, IHC, WB
Immunogen:
Recombinant protein corresponding to Met1-Ala217 of human RGS19, expressed in E. coli.
Sequence analysis predicted that GAIP is a 24.6kD, hydrophilic protein that lacks a transmembrane domain and contains multiple potential phosphorylation sites. GAIP shares approximately 31% aa identity with regulator of G protein signaling-1 (RGS1) and -2 (RGS2), the 125aa core domains of GAIP, RGS1, and RGS2 share approximately 64% homology. Northern blot analysis detected a 1.6-kb GAIP transcript in lung, placenta, liver, heart, and pancreas, with almost no expression detected in brain, skeletal muscle, and kidney. Yeast 2-hybrid binding analyses showed that through its core domain, GAIP interacts strongly with GNAI3 and weakly with GNAI2, but does not interact with GNA11. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
