Alkaline Phosphatase (ALP) BioAssay(TM) ECL Kit (Bovine)

Product Overview

• Article Name: Alkaline Phosphatase (ALP) BioAssay(TM) ECL Kit (Bovine)
• Biozol Catalog Number: USB-365697
• Supplier Catalog Number: 365697
• Alternative Catalog Number: USB-365697-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Alkaline Phosphatase (ALP) BioAssay(TM) ECL Kit is designed for the in vitro sensitive quantitative measurement of ALP in bovine serum, plasma, tissue homogenates, cell lysates, cell culture superanatants and other biological fluids. Detection Range: 41.15-30,000pg/ml Sensitivity: 25.3pg/ml Intra-Assay CV: <10% Inter-Assay CV: <12% Kit Components: *365697A: Microtiter Plate, 1x96 wells, Pre-coated with antibody, ready to use. *365697B: Standard, 2x1vial 365697C: Standard Diluent, 1x20ml *365697D: Detection Reagent A (green), 1x120ul *365697E: Detection Reagent B (red), 1x120ul 365697F: Assay Diluent A, 1x12ml 365697G: Assay Diluent B, 1x12ml 365697H: Substrate A, 1x10ml 365697J: Substrate B, 1x2ml *365697K: Positive Control, 1x1vial, xx.xpg/ml (Lyophilized) 365697L: Wash Buffer, 30X, 1x20ml Storage and Stability: Store *365697A, *365697B, *365697D, *365697E and *365697K at -20C. After reconstitution of *365697K, store at 4C and use within 5 days. Store all the other components at 4C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well,. Incubate 1 hoour at 37C. 3. Aspirate and add 100ul Detection Reagent A. Incubate 1 hour at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul Detection Reagent B. Incubate 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 100ul Substrate Working Solution. Incubate 10 minutes at 37C. 8. Read RLU values immediately.