AGA, as known as N (4)-(beta-N-acetylglucosaminyl)-L-asparaginase isoform 1, belongs to the N-terminal nucleophile (Ntn) hydrolase superfamily. This protein consists of different S100 proteins and then plays various roles in regulation of protein phosphorylation, the dynamics of cytoskeleton constituents, transcription factors, enzyme activities, cell growth and differentiation, and inflammatory response. Source: Recombinant protein corresponding to aa24-346 from human AGA, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~35.7kD (332aa) 18-57kD (SDS-PAGE under reducing conditions.) Amino Acid Sequence: ADPSSPLPLV VNTWPFKNAT EAAWRALASG GSALDAVESG CAMCEREQCD GSVGFGGSPD ELGETTLDAM IMDGTTMDVG AVGDLRRIKN AIGVARKVLE HTTHTLLVGE SATTFAQSMG FINEDLSTTA SQALHSDWLA RNCQPNYWRN VIPDPSKYCG PYKPPGILKQ DIPIHKETED DRGHDTIGMV VIHKTGHIAA GTSTNGIKFK IHGRVGDSPI PGAGAYADDT AGAAAATGNG DILMRFLPSY QAVEYMRRGE DPTIACQKVI SRIQKHFPEF FGAVICANVT GSYGAACNKL STFTQFSFMV YNSEKNQPTE EKVDCIHHHH HH Endotoxin: ~1EU/1ug (LAL) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
