Ephb4, also known as ephrin type-B receptor 4 isoform b, is a member of the Eph receptor tyrosine kinase family shown to act in neuronal guidance and mediate venal/arterial separation. Ephb4 provides survival advantage to squamous cell carcinoma of the head and neck. It has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Source: Recombinant protein corresponding to aa16-539 from mouse Ephb4, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~58.7kD (532aa) 50-70KD (SDS-PAGE under reducing conditions) Amino Acid Sequence: LEETLLNTKL ETADLKWVTY PQAEGQWEEL SGLDEEQHSV RTYEVCDMKR PGGQAHWLRT GWVPRRGAVH VYATIRFTMM ECLSLPRASR SCKETFTVFY YESEADTATA HTPAWMENPY IKVDTVAAEH LTRKRPGAEA TGKVNIKTLR LGPLSKAGFY LAFQDQGACM ALLSLHLFYK KCSWLITNLT YFPETVPREL VVPVAGSCVA NAVPTANPSP SLYCREDGQW AEQQVTGCSC APGYEAAESN KVCRACGQGT FKPQIGDESC LPCPANSHSN NIGSPVCLCR IGYYRARSDP RSSPCTTPPS APRSVVHHLN GSTLRLEWSA PLESGGREDL TYAVRCRECR PGGSCLPCGG DMTFDPGPRD LVEPWVAIRG LRPDVTYTFE VAALNGVSTL ATGPPPFEPV NVTTDREVPP AVSDIRVTRS SPSSLILSWA IPRAPSGAVL DYEVKYHEKG AEGPSSVRFL KTSENRAELR GLKRGASYLV QVRARSEAGY GPFGQEHHSQ TQLDESESWR EQLAVEHHHH HH Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
