ITGB2, also known as integrin beta-2, is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It may belong to an extended family of cell surface molecules including the fibronectin binding protein. Also It is a complement receptor type 3 (CR3). It triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation. Source: Recombinant protein corresponding to aa23-700 from human ITGB2, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~75.9kD (686aa) 70-100kD (SDS-PAGE under reducing conditions) Amino Acid Sequence: QECTKFKVSS CRECIESGPG CTWCQKLNFT GPGDPDSIRC DTRPQLLMRG CAADDIMDPT SLAETQEDHN GGQKQLSPQK VTLYLRPGQA AAFNVTFRRA KGYPIDLYYL MDLSYSMLDD LRNVKKLGGD LLRALNEITE SGRIGFGSFV DKTVLPFVNT HPDKLRNPCP NKEKECQPPF AFRHVLKLTN NSNQFQTEVG KQLISGNLDA PEGGLDAMMQ VAACPEEIGW RNVTRLLVFA TDDGFHFAGD GKLGAILTPN DGRCHLEDNL YKRSNEFDYP SVGQLAHKLA ENNIQPIFAV TSRMVKTYEK LTEIIPKSAV GELSEDSSNV VQLIKNAYNK LSSRVFLDHN ALPDTLKVTY DSFCSNGVTH RNQPRGDCDG VQINVPITFQ VKVTATECIQ EQSFVIRALG FTDIVTVQVL PQCECRCRDQ SRDRSLCHGK GFLECGICRC DTGYIGKNCE CQTQGRSSQE LEGSCRKDNN SIICSGLGDC VCGQCLCHTS DVPGKLIYGQ YCECDTINCE RYNGQVCGGP GRGLCFCGKC RCHPGFEGSA CQCERTTEGC LNPRRVECSG RGRCRCNVCE CHSGYQLPLC QECPGCPSPC GKYISCAECL KFEKGPFGKN CSAACPGLQL SNNPVKGRTC KERDSEGCWV AYTLEQQDGM DRYLIYVDES RECVAGPNLE HHHHHH Endotoxin: ~1EU/1ug (LAL) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
