Ldha, Recombinant, Rat, aa1-332, His-Tag (L-lactate Dehydrogenase A Chain)
Biozol Catalog Number:
USB-369091
Supplier Catalog Number:
369091
Alternative Catalog Number:
USB-369091-10,USB-369091-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Ldha, also known as lactate dehydrogenase A, catalyzes the conversion of pyruvate to lactate, utilizing NADH as a cofactor. It has been identified as a potential therapeutic target in the area of cancer metabolism. Reduction in Ldha activity resulted in stimulation of mitochondrial respiration and decrease of mitochondrial membrane potential. Source: Recombinant protein corresponding to aa1-332 from rat Ldha, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~37.5kD (340aa) 28-40KD (SDS-PAGE under reducing conditions) Amino Acid Sequence: MAALKDQLIV NLLKEEQVPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLKTPKIVSS KDYSVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPQ CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGV HPLSCHGWVL GEHGDSSVPV WSGVNVAGVS LKSLNPQLGT DADKEQWKDV HKQVVDSAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPISTMIKGL YGIKEDVFLS VPCILGQNGI SDVVKVTLTP DEEARLKKSA DTLWGIQKEL QFLEHHHHHH Endotoxin: ~1EU/1ug (LAL) Specific activity: >200units/mg, in which one unit will convert 1umole of pyruvate to L-lactate and beta-NAD/minute at pH 7.5 at 37C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
