Angiotensin 1-7 (Ang1-7) BioAssay(TM) ELISA Kit (Rat)

Product Overview

• Article Name: Angiotensin 1-7 (Ang1-7) BioAssay(TM) ELISA Kit (Rat)
• Biozol Catalog Number: USB-369731
• Supplier Catalog Number: 369731
• Alternative Catalog Number: USB-369731-48,USB-369731-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Angiotensin 1-7 (Ang1-7) BioAssay(TM) ELISA Kit is a quantitative sandwich assay for the detection of Ang1-7 in rat serum, plasma, tissue homogenates and other biological fluids. Detection Range: 7.813-500pg/ml Sensitivity: <4.688pg/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ang1-7. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to Ang1-7. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ang1-7, biotin-conjugated antibody and enzyme-conjugated streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of Ang1-7 in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *369731A: Microtiter Strips, 1x96 wells (8x12 wells) *369731B: Standard, 2x1 vial 369731C: Sample/Standard Dilution Buffer, 1x20ml 369731D: Antibody-Biotin (Concentrated), 1x120ul 369731E: Antibody Dilution Buffer, 1x10ml 369731F: Streptavidin-HRP Conjugate (SABC), 1x120ul 369731G: SABC Dilution Buffer, 1x10ml 369731H: TMB Substrate, 1x10ml 369731J: Stop Solution, 1x10ml 369731K: Wash Buffer, 25X, 1x30ml Storage and Stability: Store unopened *369731A at 4C, store at -20C once opened. Store unopened *369731B at 4C, once reconstituted, store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Wash plate 2 times before adding standards, samples and control (zero) to wells. 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37C. Aspirate and wash 2 times. 3. Add 100ul Antibody-Biotin working solution to each well and incubate for 60 minutes at 37C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 15-30 minutes at 37C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results.