Cytokeratin 18 (CK-18/KRT18) BioAssay(TM) ELISA Kit (Rat)

Product Overview

• Article Name: Cytokeratin 18 (CK-18/KRT18) BioAssay(TM) ELISA Kit (Rat)
• Biozol Catalog Number: USB-369820
• Supplier Catalog Number: 369820
• Alternative Catalog Number: USB-369820-48,USB-369820-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Cytokeratin 18 (CK-18/KRT18) BioAssay(TM) ELISA Kit is a quantitative sandwich assay for the detection of Cytokeratin 18 in rat serum, plasma, tissue homogenates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.094ng/ml Precision: Intra-assay CV: <8% Inter-assay CV: <10% Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytokeratin 18. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to Cytokeratin 18. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytokeratin 18, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm. The concentration of Cytokeratin 18 in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *369820A: Microtiter Strips, 8x6 wells or 8x12 wells. *369820B: Standard, 1x1vial or 2x1 vial 369820C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml 369820D: Antibody-Biotin Concentrate, 1x60ul or 1x120ul 369820E: Antibody Dilution Buffer, 1x5ml or. 1x10ml *369820F: Streptavidin-HRP Conjugate (SABC), 1x60ul or 1x120ul 369820G: SABC Dilution Buffer, 1x5ml or 1x10ml *369820H: TMB Substrate, 1x5ml or 1x10ml 369820J: Stop Solution, 1x5ml or 1x10ml 369820K: Wash Buffer, 25X, 1x15ml or 1x30ml Storage and Stability: Store unopened *369820A at 4C, store at -20C once opened. Store unopened *369820B at 4C, once reconstituted store at 4C for up to 12 hours or at -20C for up to 48 hours. Store *369820F and *369820H at 4C in the dark. Store other components at 4C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Wash plate 2 times before adding standards and samples to wells! 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37C. Aspirate and wash 2 times. 3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results.