Heparan Sulfate BioAssay(TM) ELISA Kit (Mouse)

Product Overview

• Article Name: Heparan Sulfate BioAssay(TM) ELISA Kit (Mouse)
• Biozol Catalog Number: USB-369957
• Supplier Catalog Number: 369957
• Alternative Catalog Number: USB-369957-48,USB-369957-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Heparan Sulfate BioAssay(TM) ELISA Kit is a quantitative competitive assay for the detection of Heparan Sulfate in mouse serum, plasma, tissue homogenates and other biological fluids. Detection Range: 31.25-2000ng/ml Sensitivity: 18.75ng/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Assay Principle: The microtiter plate provided in this kit has been pre-coated with Heparan Sulfate. Heparan Sulfate in the standard or sample competes with a fixed amount of plate-coated Heparan Sulfate for antibody binding sites on the Antibody-Biotin reagent. Excess conjugate and unbound sample or standard are washed from the plate, after which Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. TMB substrate is then added to each well. The enzyme-substrate reaction is terminated by the addition of a sulfuric acid solution and the resulting color is measured spectrophotometrically at 450nm. The concentration of Heparan Sulfate in the samples is determined by an inverse correlation between Heparan Sulfate in the sample and the assay signal intensity. Kit Components: *369957A: Microtiter Strips, 1x48 wells or 1x96 wells *369957B: Standard, 1x1 vial or 2x1 vial 369957C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml 369957D: Antibody-Biotin (Concentrated), 1x30ul or 1x60ul 369957E: Antibody Dilution Buffer, 1x5ml or 1x10ml 369957F: Streptavidin (HRP) (SABC, 1x60ul or 1x120ul 369957G: SABC Dilution Buffer, 1x5ml or 1x10ml 369957H: TMB Substrate, 1x5ml or 1x10ml 369957J: Stop Solution, 1x5ml or 1x10ml 369957K: Wash Buffer, 25X, 1x15ml or 1x30ml Storage and Stability: Store unopened *369957A at 4C, store at -20C once opened. Store unopened *369957B at 4C, once reconstituted, store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Wash plate 2 times before adding standards and samples to wells! 2. Add 50ul standard or sample to each well. 3. Add 50ul Antibody (Biotin) working solution to each well and incubate for 45 minutes at 37C 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results