Tumor Necrosis Factor-alpha (TNF-alpha) BioAssay(TM) ELISA Kit (Hamster)

Product Overview

• Article Name: Tumor Necrosis Factor-alpha (TNF-alpha) BioAssay(TM) ELISA Kit (Hamster)
• Biozol Catalog Number: USB-370268
• Supplier Catalog Number: 370268
• Alternative Catalog Number: USB-370268-48,USB-370268-96
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

The Tumor Necrosis Factor-alpha (TNF-alpha) BioAssay(TM) ELISA Kit is a quantitative sandwich assay for the detection of TNF-alpha in hamster serum, plasma, tissue homogenates and other biological fluids. Detection Range: 3.125-200pg/ml Sensitivity: 1.875pg/ml Precision: Intra-Assay: CV<8% Inter-Assay: CV<10% Assay Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNF-alpha. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to TNF-alpha. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TNF-alpha, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm 10nm. The concentration of TNF-alpha in the sample is then determined by comparing the O.D. of the sample to the standard curve. Kit Components: *370268A: Microtiter Strips, 1x48 wells or 1x96 wells *370268B: Standard, 1x1vial or 2x1 vial 370268C: Sample/Standard Dilution Buffer, 1x10ml or 1x20ml 370268D: Antibody (Biotin) (Concentrated), 1x60ul or 1x120ul 370268E: Antibody Dilution Buffer, 1x5ml or 1x10ml 370268F: Streptavidin (HRP) (SABC), 1x60ul or 1x120ul 370268G: SABC Dilution Buffer, 1x5ml or 1x10ml 370268H: TMB Substrate, 1x5ml or 1x10ml 370268J: Stop Solution, 1x5ml or 1x10ml 370268K: Wash Buffer, 25X, 1x15ml or 1x30ml Storage and Stability: Store unopened *370268A at 4C, store at -20C once opened. Store unopened *370268B at 4C, once reconstituted, store at 4C for up to 12 hours or at -20C for up to 48 hours. Store other components at 4C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Summary: 1. Wash plate 2 times before adding standards and samples to wells! 2. Add 100ul standard or sample to each well and incubate for 90 minutes at 37C. Aspirate and wash 2 times. 3. Add 100ul Antibody (Biotin) working solution to each well and incubate for 60 minutes at 37C. 4. Aspirate and wash 3 times. 5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37C 6. Aspirate and wash 5 times. 7. Add 90ul TMB substrate. Incubate 10-20 minutes at 37C 8. Add 50ul Stop Solution. Read at 450nm immediately. 9. Calculate results