Apolipoprotein E, Recombinant, Rabbit, aa19-311, His-Tag (APOE)
Biozol Catalog Number:
USB-370528
Supplier Catalog Number:
370528
Alternative Catalog Number:
USB-370528-20,USB-370528-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Source: Recombinant protein corresponding to aa19-311 from rabbit APOE, fused to His-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli. Amino Acid Sequence: QT EQEVEVPEQA RWKAGQPWEL ALGRFWDYLR WVQSLSDQVQ EELLSSQVTQ ELTMLMEETM KEVKAYKSEL EEQLSPMAQE HRARLSKELQ VAGALEADME DVCNRLAQYR GEAQAMLGQS TEELARAFSS HLRKLRKRLL RDAEDLQKRM AVYGAGAREG AERGVSAVRE RLGSRLERGR LRVATVGTLA GRPLRERAQA WGERLRGHLE EVGSRARDRL NEVREQVEEV RVKVEEQAPQ MRLQAEAFQA RLKSWFEPLV EDMQRQWAGL VEKLQAAMPS KAPAAAPIEN Q Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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