Glutamate-cysteine Ligase, Recombinant, E. coli, aa1-518, His-SUMO-Tag (GshA)

Product Overview

• Article Name: Glutamate-cysteine Ligase, Recombinant, E. coli, aa1-518, His-SUMO-Tag (GshA)
• Biozol Catalog Number: USB-370630
• Supplier Catalog Number: 370630
• Alternative Catalog Number: USB-370630-20,USB-370630-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Full length recombinant protein corresponding to aa1-518 from Escherichia coli gshA, fused to 6X His-SUMO-Tag at N-terminal, expressed in E. coli. Swiss/Uniprot: P0A6W9 Molecular Weight: ~74.23kD Amino Acid Sequence: MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGHPEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPLSMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFWQAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPFEKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAKIGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLGIGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSARILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTEPFAVWLEKHA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 74.23
• UniProt: P0A6W9
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.