HupA, Recombinant, E. Coli, aa1-90, His-SUMO-Tag (DNA-binding Protein HU-alpha)
Biozol Catalog Number:
USB-370650
Supplier Catalog Number:
370650
Alternative Catalog Number:
USB-370650-20,USB-370650-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions. Source: Recombinant protein corresponding to aa1-90 from full length E. coli hupA, fused to His-SUMO-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~25.53kD Amino Acid Sequence: MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQLVGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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