ATP-dependent DNA helicase PcrA, Recombinant, Geobacillus Stearothermophilus, aa10-289, His-Tag (PcrA)
Biozol Catalog Number:
USB-372374
Supplier Catalog Number:
372374
Alternative Catalog Number:
USB-372374-20,USB-372374-100
Manufacturer:
US Biological
Category:
Molekularbiologie
DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3-5 helicase but at high protein concentrations it can also displace a substrate with a 5 tail. Preferred substrate being one with both single and double-stranded regions of DNA. Partial recombinant protein corresponding to aa10-289 from Geobacillus stearothermophilus ATP-dependent DNA helicase PcrA, fused to 6X His-Tag at N-terminal, expressed in Yeast. Swiss/Uniprot: P56255 Molecular Weight: ~34.6kD Amino Acid Sequence: AHLNKEQQEAVRTTEGPLLIMAGAGSGKTRVLTHRIAYLMAEKHVAPWNILAITFTNKAAREMRERVQSLLGGAAEDVWISTFHSMCVRILRRDIDRIGINRNFSILDPTDQLSVMKTILKEKNIDPKKFEPRTILGTISAAKNELLPPEQFAKRASTYYEKVVSDVYQEYQQRLLRNHSLDFDDLIMTTIQLFDRVPDVLHYYQYKFQYIHIDEYQDTNRAQYTLVKKLAERFQNICAVGDADQSIYRWRGADIQNILSFERDYPNAKVILLEQNYRST Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
