CAPZA2, Recombinant, Human, aa2-286, His-SUMO-Tag (F-actin-capping Protein Subunit Alpha-2)

Catalog Number: USB-372567
Article Name: CAPZA2, Recombinant, Human, aa2-286, His-SUMO-Tag (F-actin-capping Protein Subunit Alpha-2)
Biozol Catalog Number: USB-372567
Supplier Catalog Number: 372567
Alternative Catalog Number: USB-372567-20,USB-372567-100,USB-372567-1
Manufacturer: US Biological
Category: Molekularbiologie
F-actin-capping proteins bind in a Ca2+-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. Source: Recombinant protein corresponding to aa2-286 from human CAPZA2, fused to His-SUMO-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~48.8kD Amino Acid Sequence: ADLEEQLSDEEKVRIAAKFIIHAPPGEFNEVFNDVRLLLNNDNLLREGAAHAFAQYNLDQFTPVKIEGYEDQVLITEHGDLGNGKFLDPKNRICFKFDHLRKEATDPRPCEVENAVESWRTSVETALRAYVKEHYPNGVCTVYGKKIDGQQTIIACIESHQFQAKNFWNGRWRSEWKFTITPSTTQVVGILKIQVHYYEDGNVQLVSHKDIQDSLTVSNEVQTAKEFIKIVEAAENEYQTAISENYQTMSDTTFKALRRQLPVTRTKIDWNKILSYKIGKEMQNA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight: 48.8
UniProt: P47755
Purity: 85% (SDS-PAGE)
Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.