Ctdp1, Recombinant, Mouse, aa178-341, His-Tag, Myc-Tag (RNA Polymerase II Subunit A C-terminal Domain Phosphatase)

Product Overview

• Article Name: Ctdp1, Recombinant, Mouse, aa178-341, His-Tag, Myc-Tag (RNA Polymerase II Subunit A C-terminal Domain Phosphatase)
• Biozol Catalog Number: USB-372902
• Supplier Catalog Number: 372902
• Alternative Catalog Number: USB-372902-20,USB-372902-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Processively dephosphorylates Ser-2 and Ser-5 of the heptad repeats YSPTSPS in the C-terminal domain of the largest RNA polymerase II subunit. This promotes the activity of RNA polymerase II. Plays a role in the exit from mitosis by dephosphorylating crucial mitotic substrates (USP44, CDC20 and WEE1) that are required for M-phase-promoting factor (MPF)/CDK1 inactivation. Source: Partial recombinant protein corresponding to aa178-341 from mouse Ctdp1, fused to 10xHis-Tag at N-terminal and Myc-Tag at C-terminal, expressed in E. coli. Molecular Weight: ~24kD Amino Acid Sequence: HRNRKLVLMVDLDQTLIHTTEQHCPQMSNKGIFHFQLGRGEPMLHTRLRPHCKDFLEKIAKLYELHVFTFGSRLYAHTIAGFLDPEKKLFSHRILSRDECIDPFSKTGNLRNLFPCGDSMVCIIDDREDVWKFAPNLITVKKYVYFPGTGDVNAPPAARETQAR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 24
• UniProt: Q7TSG2
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.