Def, Recombinant, E. coli, aa2-169, His-Tag (Peptide Deformylase)

Product Overview

• Article Name: Def, Recombinant, E. coli, aa2-169, His-Tag (Peptide Deformylase)
• Biozol Catalog Number: USB-373020
• Supplier Catalog Number: 373020
• Alternative Catalog Number: USB-373020-20,USB-373020-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Roves the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions. Source: Recombinant protein corresponding to aa2-169 from E. coli Peptide Deformylase, fused to His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~21.2kD Amino Acid Sequence: SVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEGIGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVPRAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVEKLDRLKARA Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 21.2
• UniProt: P0A6K3
• Purity: 85% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.