E2, Recombinant, Human, aa1-365, GST-Tag (Papillomavirus Type 16 Regulatory Protein E2)

Product Overview

• Article Name: E2, Recombinant, Human, aa1-365, GST-Tag (Papillomavirus Type 16 Regulatory Protein E2)
• Biozol Catalog Number: USB-373116
• Supplier Catalog Number: 373116
• Alternative Catalog Number: USB-373116-20,USB-373116-100,USB-373116-1
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Plays an accessory role in initiation of DNA replication. A dimer of E2 interacts with a dimer of E1 in order to improve specificity of E1 DNA binding activity. Once the complex recognizes and binds DNA at specific sites, the E2 dimer is roved from DNA. E2 also regulates viral transcription through binding to the E2RE response element (5-ACCNNNNNNGGT-3) present in multiple copies in the regulatory regions of the viral genome. Activates or represses transcription depending on E2REs position with regards to proximal promoter elements including the TATA-box. Repression occurs by sterically hindering the assembly of the transcription initiation complex. Source: Recombinant protein corresponding to aa1-365 from human E2, fused to GST-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~68.8kD Amino Acid Sequence: METLCQRLNVCQDKILTHYENDSTDLRDHIDYWKHMRLECAIYYKAREMGFKHINHQVVPTLAVSKNKALQAIELQLTLETIYNSQYSNEKWTLQDVSLEVYLTAPTGCIKKHGYTVEVQFDGDICNTMHYTNWTHIYICEEASVTVVEGQVDYYGLYYVHEGIRTYFVQFKDDAEKYSKNKVWEVHAGGQVILCPTSVFSSNEVSSPEIIRQHLANHPAATHTKAVALGTEETQTTIQRPRSEPDTGNPCHTTKLLHRDSVDSAPILTAFNSSHKGRINCNSNTTPIVHLKGDANTLKCLRYRFKKHCTLYTAVSSTWHWTGHNVKHKSAIVTLTYDSEWQRDQFLSQVKIPKTITVSTGFMSI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 68.8
• UniProt: P03120
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.