Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by up-regulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2/M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells (By similarity). Source: Partial recombinant protein corresponding to aa247-454 of Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4) Epstein-Barr Nuclear Antigen 2, fused to His-Tag at N-terminal, expressed in Yeast. Amino Acid Sequence: MPTYYLALHG GQSYNLIVDT DMSGNPSLSV IPTNPYQEQL SNNPLIQLQI VVGENTGAPA PPQPPPPPPP PPPPERRDAW TQEPLPLDMN PLGSDASQGP LASSIRMLCM AQYLLRNARG QQGLLRPLGP QTRSQVTLER QPVHNPRQEA PIILLQSPAP PRFTPVPMVA LGHTLQPTPP PRPTLPQPRI PLIIPPRHTN QPATTPPTAP QRLTLGHQLS LPPHPPPHQS TPHCSSDSTG LPPPPTSYSI PSMTLSPEPL PPPAAPAHPL PGVIYDQQAL PPTPGPPWWP PVRDPTPTTQ TPPTNTKQGP DQGQGRGRWR GRGRSKGRGR MHKLPEPRRP GPDTSSPSMP QLSPVVSLHQ GQGPENSPTP GPSTAGPVCR VTPSATPDIS PIHEPESSDS EEPPFLFPSD WYPPTLEPAE LDESWEGIFE TTESHSSDEE NVGGPSKRPR TSTQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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