Fbxw10, Recombinant, Mouse, aa701-1030, His-SUMO-Tag (F-box/WD Repeat-containing Protein 10)

Product Overview

• Article Name: Fbxw10, Recombinant, Mouse, aa701-1030, His-SUMO-Tag (F-box/WD Repeat-containing Protein 10)
• Biozol Catalog Number: USB-373292
• Supplier Catalog Number: 373292
• Alternative Catalog Number: USB-373292-20, USB-373292-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Probable substrate-recognition component of a SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Overexpression is leading to degradation of CBX5 and CBX1 (By similarity). Source: Recombinant protein corresponding to aa701-1030 from mouse Fbxw10, fused to His-SUMO-Tag at N-terminal expressed in E. coli. Molecular Weight: ~53.2kD Amino Acid Sequence: VKWQYSSDKNKVKKSKDKEEEREETSLGDEHSRSTIQGHSLKDSVSSKQEFSKSRVHLKQTKNLSSDDMETPVGEVSHPLQKLWKVPMTPDRFLLTISALQQAHNSEEFAYPHRPRPQVIDAWGPSIPYPRKVLSLKGKSVQHAVDQLRSSNLPTGVRQTNIPLEIQKLQPNLKKSLHSPRVQATVPQPSLIRPKVSDSLRGDEHLTSSIDGTMRRAGPLTSMQVIKPNRMLAPRGGTATLSPKKERPRFYTTLDPLRMNTGFMLMTVKEEKEFAEAKMKEYEASVSTKEVDPGKASKAAWIRKIKGLPIDNFMKEGKTAAPELGQNVFI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 53.2
• UniProt: Q5SUS0
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.