Lactose Operon Repressor, Recombinant, E. coli, aa1-360, His-SUMO-Tag (LacI)
Biozol Catalog Number:
USB-373978
Supplier Catalog Number:
373978
Alternative Catalog Number:
USB-373978-20,USB-373978-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Repressor of the lactose operon. Binds allolactose as an inducer. Recombinant protein corresponding to aa1-360 from full length E. coli Lactose Operon Repressor, fused to His-SUMO-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~54.6kD Amino Acid Sequence: MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
* VAT and and shipping costs not included. Errors and price changes excepted