MutT, Recombinant, E. coli, aa1-129, His-Tag (8-oxo-dGTP Diphosphatase)

Product Overview

• Article Name: MutT, Recombinant, E. coli, aa1-129, His-Tag (8-oxo-dGTP Diphosphatase)
• Biozol Catalog Number: USB-374332
• Supplier Catalog Number: 374332
• Alternative Catalog Number: USB-374332-20,USB-374332-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus leading to A.T to G.C transversions. MutT specifically degrades 8-oxo-dGTP to the monophosphate. Source: Full length recombinant protein corresponding to aa1-129 from E. coli mutT, fused to His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~16.9kD Amino Acid Sequence: MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGETPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVERWEGEPWGKEGQPGEWMSLVGLNADDFPPANEPVIAKLKRL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 16.9
• UniProt: P08337
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.