NCK2, Recombinant, Human, aa1-380, His-SUMO-Tag (Cytoplasmic Domain Protein NCK2)

Product Overview

• Article Name: NCK2, Recombinant, Human, aa1-380, His-SUMO-Tag (Cytoplasmic Domain Protein NCK2)
• Biozol Catalog Number: USB-374380
• Supplier Catalog Number: 374380
• Alternative Catalog Number: USB-374380-20,USB-374380-100,USB-374380-1
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Source: Recombinant protein corresponding to aa1-380 from human NCK2, fused to His-SUMO-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~58.9kD Amino Acid Sequence: MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 58.9
• UniProt: O43639
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.