NfsA, Recombinant, E. coli, aa1-240, His-Tag (Oxygen-insensitive NADPH Nitroreductase)

Product Overview

• Article Name: NfsA, Recombinant, E. coli, aa1-240, His-Tag (Oxygen-insensitive NADPH Nitroreductase)
• Biozol Catalog Number: USB-374410
• Supplier Catalog Number: 374410
• Alternative Catalog Number: USB-374410-20,USB-374410-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Reduction of nitroaromatic compounds using NADH. Reduces nitrofurazone by a ping-pong bi-bi mechanism possibly to generate a two-electron transfer product. Major component of the oxygen-insensitive nitroreductase activity in E.coli. Source: Recombinant protein corresponding to aa1-240 from E. coli nfsA, fused to His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~28.8kD Amino Acid Sequence: MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQCSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLLLGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWPADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRRTIIKESRPFILDYLHKQGWATR Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 28.8
• UniProt: P17117
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.