PcrA, Recombinant, Geobacillus Stearothermophilus, aa10-289, His-SUMO-Tag (ATP-dependent DNA helicase PcrA)

Product Overview

• Article Name: PcrA, Recombinant, Geobacillus Stearothermophilus, aa10-289, His-SUMO-Tag (ATP-dependent DNA helicase PcrA)
• Biozol Catalog Number: USB-374634
• Supplier Catalog Number: 374634
• Alternative Catalog Number: USB-374634-20,USB-374634-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3-5 helicase but at high protein concentrations it can also displace a substrate with a 5 tail. Preferred substrate being one with both single and double-stranded regions of DNA. Partial recombinant protein corresponding to aa10-289 from geobacillus stearothermophilus PcrA, fused to His-SUMO-Tag at N-terminal, expressed in E. coli. Swiss/Uniprot: P56255 Molecular Weight: ~48.6kD Amino Acid Sequence: AHLNKEQQEAVRTTEGPLLIMAGAGSGKTRVLTHRIAYLMAEKHVAPWNILAITFTNKAAREMRERVQSLLGGAAEDVWISTFHSMCVRILRRDIDRIGINRNFSILDPTDQLSVMKTILKEKNIDPKKFEPRTILGTISAAKNELLPPEQFAKRASTYYEKVVSDVYQEYQQRLLRNHSLDFDDLIMTTIQLFDRVPDVLHYYQYKFQYIHIDEYQDTNRAQYTLVKKLAERFQNICAVGDADQSIYRWRGADIQNILSFERDYPNAKVILLEQNYRST Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 48.6
• UniProt: P56255
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.