PTH, Recombinant, E. coli, aa1-194, His-Tag (Peptidyl-tRNA Hydrolase)
Biozol Catalog Number:
USB-374922
Supplier Catalog Number:
374922
Alternative Catalog Number:
USB-374922-20, USB-374922-100
Manufacturer:
US Biological
Category:
Molekularbiologie
The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. Involved in lambda inhibition of host protein synthesis. PTH activity may, directly or indirectly, be the target for lambda bar RNA leading to rap cell death. Source: Recombinant protein corresponding to aa1-194 from E. coli Peptidyl-tRNA Hydrolase, fused to His-Tag at N-terminal expressed in E. coli. Molecular Weight: ~25.1kD Amino Acid Sequence: MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAKFFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGVAKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQKLIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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