RAB5A, Recombinant, Human, aa1-215, His-SUMO-Tag (Ras-related Protein Rab-5A)

Product Overview

• Article Name: RAB5A, Recombinant, Human, aa1-215, His-SUMO-Tag (Ras-related Protein Rab-5A)
• Biozol Catalog Number: USB-374973
• Supplier Catalog Number: 374973
• Alternative Catalog Number: USB-374973-20,USB-374973-100,USB-374973-1
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Source: Recombinant protein corresponding to aa1-215 from human RAB5A, fused to His-SUMO-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~39.7kD Amino Acid Sequence: MASRGATRPNGPNTGNKICQFKLVLLGESAVGKSSLVLRFVKGQFHEFQESTIGAAFLTQTVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNEESFARAKNWVKELQRQASPNIVIALSGNKADLANKRAVDFQEAQSYADDNSLLFMETSAKTSMNVNEIFMAIAKKLPKNEPQNPGANSARGRGVDLTEPTQPTRNQCCSN Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 39.7
• UniProt: P20339
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.