RAN, Recombinant, Human, aa1-216, GST-Tag (GTP-Binding Nuclear Protein Ran)

Product Overview

• Article Name: RAN, Recombinant, Human, aa1-216, GST-Tag (GTP-Binding Nuclear Protein Ran)
• Biozol Catalog Number: USB-374991
• Supplier Catalog Number: 374991
• Alternative Catalog Number: USB-374991-20,USB-374991-100,USB-374991-1
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensure the directionality of the transport. Source: Recombinant protein corresponding to aa1-216 from human RAN, fused to GST-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~51.3kD Amino Acid Sequence: AAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 51.3
• UniProt: P62826
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.