RplF, Recombinant, E. coli, aa2-175, GST-Tag (50S Ribosomal Protein L6)

Product Overview

• Article Name: RplF, Recombinant, E. coli, aa2-175, GST-Tag (50S Ribosomal Protein L6)
• Biozol Catalog Number: USB-375110
• Supplier Catalog Number: 375110
• Alternative Catalog Number: USB-375110-20,USB-375110-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

This protein binds directly to at least 2 domains of the 23S ribosomal RNA, thus is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.Gentamicin-resistant mutations in this protein affect translation fidelity. Source: Recombinant protein corresponding to aa2-175 from E. coli 50S Ribosomal Protein L6, fused to GST-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~45.5kD Amino Acid Sequence: SRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVKHADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNVINLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGKGVRYADEVVRTKEAK Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 45.5
• UniProt: P0AG55
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.