RtcB, Recombinant, E. coli, aa1-408, His-Tag (RNA-splicing Ligase RtcB)
Biozol Catalog Number:
USB-375167
Supplier Catalog Number:
375167
Alternative Catalog Number:
USB-375167-20,USB-375167-100
Manufacturer:
US Biological
Category:
Molekularbiologie
RNA ligase that mediates the joining of broken tRNA-like stem-loop structures in case of tRNA damage. Probably participates to tRNA restriction-repair by ligating broken tRNA-like stem-loop structures with 2,3-cyclic phosphate and 5-OH ends to form a splice junction with a 2-OH, 3,5-phosphodiester, a step that requires GTP. Also acts as a DNA ligase in case of DNA damage by splicing dirty DNA breaks, characterized by 3-PO4 (or cyclic-PO4) and 5-OH ends that cannot be sealed by classical DNA ligases. Source: Recombinant protein corresponding to aa1-408 from E. coli rtcB, fused to His-Tag at N-terminal, expressed in Yeast. Molecular Weight: ~47.2kD Amino Acid Sequence: MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIAVMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQAIETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHLGTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRDLAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVEVIYTLRQVVCVKG Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
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