RuvA, Recombinant, E. coli, aa1-203, His-SUMO-Tag (Holliday Junction ATP-dependent DNA Helicase RuvA)

Product Overview

• Article Name: RuvA, Recombinant, E. coli, aa1-203, His-SUMO-Tag (Holliday Junction ATP-dependent DNA Helicase RuvA)
• Biozol Catalog Number: USB-375169
• Supplier Catalog Number: 375169
• Alternative Catalog Number: USB-375169-20,USB-375169-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB. Binds both single- and double-stranded DNA (dsDNA). Binds preferentially to supercoiled rather than to relaxed dsDNA. Full length recombinant protein corresponding to aa1-203 from E. coli ruvA, fused to His-SUMO-Tag at N-terminal expressed in E. coli. Molecular Weight: ~38.1kD Amino Acid Sequence: MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQEAIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAVEREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAEQEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 38.1
• UniProt: P0A809
• Purity: ~90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.