Vpx, Recombinant, Human, aa1-112, His-SUMO-Tag (Immunodeficiency Virus Type 2 Subtype A Protein Vpx)

Product Overview

• Article Name: Vpx, Recombinant, Human, aa1-112, His-SUMO-Tag (Immunodeficiency Virus Type 2 Subtype A Protein Vpx)
• Biozol Catalog Number: USB-375845
• Supplier Catalog Number: 375845
• Alternative Catalog Number: USB-375845-20,USB-375845-100
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Plays a role in nuclear translocation of the viral pre-integration complex (PIC), thus is required for the virus to infect non-dividing cells. Targets specific host proteins for degradation by the 26S proteasome. Acts by associating with the cellular CUL4A-DDB1 E3 ligase complex through direct interaction with host VPRPB/DCAF-1. This change in the E3 ligase substrate specificity results in the degradation of host SAMHD1. In turn, SAMHD1 depletion allows viral replication in host myeloid cells by preventing SAMHD1-mediated hydrolysis of intracellular dNTPs necessary for reverse transcription (By similarity). Source: Recombinant protein corresponding to aa1-112 from human vpx, fused to His-SUMO-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~28.8kD Amino Acid Sequence: MTDPRETVPPGNSGEETIGEAFAWLNRTVEAINREAVNHLPRELIFQVWQRSWRYWHDEQGMSESYTKYRYLCIIQKAVYMHVRKGCTCLGRGHGPGGWRPGPPPPPPPGLV Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 28.8
• UniProt: P06939
• Purity: 90% (SDS-PAGE)
• Form: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.