Aflatoxin M1 (AFM1) ELISA BioAssay(TM) Kit

Product Overview

• Article Name: Aflatoxin M1 (AFM1) ELISA BioAssay(TM) Kit
• Biozol Catalog Number: USB-376891
• Supplier Catalog Number: 376891
• Alternative Catalog Number: USB-376891-48,USB-376891-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Aflatoxin M1 is one of Aflatoxin with similar structure compounds. On damp-heat areas, the occurrence of Aflatoxin is highest in food and feed. Aflatoxin M1 with quite stable physical and chemistry properties, is not destroyed by pasteurization. When the mammal animal ingest food or feed containing Aflatoxin B1, it can be converted to the Aflatoxin M1 by hydroxylation. The main harm of Aflatoxin M1 is carcinogenicity and mutagenicity, destroying human and animal liver tissue, resulting in hepatocarcinoma and even death. This BioAssay(TM) kit is a detection product developed based on ELISA technology, with operation time as short as 50 minutes. Sample Type: Milk powder 1ppb, Milk 0.1ppb Intended Use: This BioAssay(TM) kit allows for the in vitro quantitative determination of Aflatoxin M1 concentrations in milk, milk power. Sensitivity: 0.02ppb Range: 0.02 ppb-1.62ppb Precision: Intra-assay: CV%<10% Inter-assay: CV%<10% Test Principle: This BioAssay(TM) employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay(TM) kit has been pre-coated with Aflatoxin M1 antigen. Standards or samples are added to the appropriate microtiter plate wells with Aflatoxin M1 specific antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Aflatoxin M1 and Aflatoxin M1 in standards or samples with the Aflatoxin M1 special antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Aflatoxin M1 in the standards or samples. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate, 96T/48T Standard (96T), 6x1ml Standard (48T), 6x0.5ml HRP-conjugate (96T), 1x7ml HRP-conjugate (48T), 1x3.5ml Antibody (96T), 1x7ml Antibody (48T), 1x1x3.5ml TMB Substrate (96T), 1x12ml TMB Substrate (48T), 1x6ml Stop Solution (96T), 1x10ml Stop Solution, (48T), 1x5ml Wash Buffer (10X) (96T), 1x50ml Wash Buffer (10X) (48T), 1x25ml Adhesive Strip (96T/48T), 4x strips Instruction Manual (96T/48T), 1x manual Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.