Deoxynivalenol (DON) ELISA BioAssay(TM) Kit

Product Overview

• Article Name: Deoxynivalenol (DON) ELISA BioAssay(TM) Kit
• Biozol Catalog Number: USB-376895
• Supplier Catalog Number: 376895
• Alternative Catalog Number: USB-376895-48,USB-376895-96
• Manufacturer: US Biological
• Category: Kits/Assays
• Application: ELISA

Product Description

Deoxynivalenol (DON), also known as Vomitoxin, belongs to trichothecene and is produced by mould fusarium, growing in grains such as wheat, maize, barley and stover. Because of causing vomitting in porcines, hence the name is Vomitoxin. It has some harmful effects to human body and has been listed as Category III carcinogen by EU classification criteria. In 2011, the limit of Deoxynivalenol residues is 1000ug/kg in grains and grains products in China. This BioAssay(TM) kit is a detection product developed based on ELISA technology, with operation time as short as 15 minutes. Sample Type: Feed, soybean meal Intended Use: This BioAssay(TM) kit allows for the in vitro quantitative determination of Deoxynivalenol concentrations in feed, soybean meal. Sensitivity: 1ug/kg Range: 1-81ug/kg Precision: Intra-assay: CV%<10% Inter-assay: CV%<10% Test Principle: This BioAssay(TM) employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this BioAssay(TM) kit has been pre-coated with Deoxynivalenol antigen. Standards or samples are added to the appropriate microtiter plate wells with Deoxynivalenol antibody and Horseradish Peroxidase (HRP) conjugated anti-antibody. The competitive inhibition reaction is launched between pre-coated Deoxynivalenol and Deoxynivalenol in standards or samples with the Deoxynivalenol antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of Deoxynivalenol in the standards or samples. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate, 96T/48T Standard (96T), 6x1ml Standard (48T), 6x0.5ml HRP-conjugate (96T), 1x7ml HRP-conjugate (48T), 1x3.5ml Antibody (96T), 1x7ml Antibody (48T), 1x1x3.5ml TMB Substrate (96T), 1x12ml TMB Substrate (48T), 1x6ml Stop Solution (96T), 1x10ml Stop Solution, (48T), 1x5ml Sample Diluent (96T), 1x50ml Sample DIluent (48T), 1x25ml Wash Buffer (10X) (96T), 1x50ml Wash Buffer (10X) (48T), 1x25ml Adhesive Strip (96T/48T), 4x strips Instruction Manual (96T/48T), 1x manual Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.